Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

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• 作者：Katayama ; Seiichi ; Matsushita ; Osamu ; Tamai ; Eiji ; Miyata ; Shigeru ; Okabe ; Akinobu
• 关键词：Curved DNA ; α ; Subunit ; RNA polymerase ; Gene expression ; Phospholipase C ; Clostridium perfringens ; PCR ; polymerase chain reaction ; RNAP ; RNA polymerase ; RNAPα ; α ; subunit of RNA polymerase ; α ; CTD ; C-terminal domain of α ; subunit ; ^
• 刊名：FEBS Letters
• 出版年：2001
• 出版时间：December 7, 2001
• 年：2001
• 卷：509
• 期：2
• 页码：235-238
• 全文大小：164 K

文摘

Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.