Linear motifs in the C-terminus of D. melanogaster cryptochrome
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- 作者：Matthew J. Hemsley ; Gabriella M. Mazzotta ; Moyra Mason ; Stephane Dissel ; Stefano Toppo ; Mario A. Pagano ; Federica S ; relli ; Flavio Meggio ; Ezio Rosato ; Rodolfo Costa ; et al.
- 关键词：Cryptochrome ; Circadian clock ; Linear motifs
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：6 April 2007
- 年：2007
- 卷：355
- 期：2
- 页码：531-537
- 全文大小：683 K
文摘
The C-terminus of cryptochrome (CRY) regulates light responses in Drosophila. These include the light-dependent binding of Drosophila dCRY to the clock proteins PERIOD and TIMELESS in a yeast two-hybrid system, which we proved to be a convenient and reliable readout of the behavior of dCRY in vivo. In this study, we present a combination of in silico analysis and experimental validation in yeast, to identify novel functional motifs in the C-terminal region of dCRY. Our results suggest that linear motifs are present in this small region, which is a likely hotspot for molecular interactions.