Structural Basis of Arp2/3 Complex Inhibition by GMF, Coronin, and Arpin

详细信息    查看全文

• 作者：Olga S. Sokolova¹ ; Angelina Chemeris¹ ; ⁵ ; Siyang Guo² ; Salvatore L. Alioto² ; Meghal Gandhi² ; Shae Padrick³ ; Evgeniya Pechnikova⁴ ; Violaine David⁵ ; Alexis Gautreau⁵ ; Bruce L. Goode² ; ^{goode@brandeis.edu}
• 关键词：Arp ; actin-related protein ; NPF ; nucleation-promoting factor ; GFP ; green fluorescent protein ; GMF ; glia maturation factor ; EM ; electron microscopy ; GST ; glutathione S-transferase ; FSC ; Fourier shell correlation ; TEV ; tobacco etch virus ; VCA ; verproline central acidic ; WASP ; Wiskott Aldrich syndrome protein ; WAVE ; WASP family verproline-homologous
• 刊名：Journal of Molecular Biology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：429
• 期：2
• 页码：237-248
• 全文大小：2482 K
• 卷排序：429

文摘

Coronin, GMF, and Arpin each induce related open conformations in Arp2/3 complex. GMF binding induces two distinct inhibitory states of Arp2/3 complex. Coronin, GMF, and Arpin combinatorially inhibit Arp2/3 complex activity. Arpin has two separate binding sites on Arp2/3 complex Arp2 and Arp3.