Lipases from the genus Rhizopus: Characteristics, expression, protein engineering and application

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• 作者：Xiao-Wei Yu^a ; ^b ; ^{yuxw@jiangnan.edu.cn} ; Yan Xu^a ; ^b ; ^{yxu@jiangnan.edu.cn} ; Rong Xiao^c
• 关键词：ROL ; R. oryzae lipase ; RCL ; R. chinensis lipase ; RSL ; R. stolonifer lipase ; RML ; Rhizomucor miehei lipase ; mROL ; the mature R. oryzae lipase ; ProROL ; the R. oryzae prolipase ; r28ROL ; the mature R. oryzae lipase containing 28 amino acids of the prosequence ; mRCL ; the mature R. chinensis lipase ; r27RCL ; the mature R. chinensis lipase containing 27 amino acids of the prosequence ; AnFaeA ; ferulic acid esterase from Aspergillus niger ; SSF ; solid-state fermentation ; SmF ; submerged fermentation ; PDB ; P
• 刊名：Progress in Lipid Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：64
• 期：Complete
• 页码：57-68
• 全文大小：974 K
• 卷排序：64

文摘

Lipases are versatile catalysts that hydrolyze ester bonds of water-insoluble glycerides or carry out reversible reactions at the water/lipid interface. The remarkable characteristics of lipases from the genus Rhizopus are their high sn-1,3-positional specificity, enantioselectivity and activity in nonaqueous media, which make them one of the most desirable enzymes for many applications, including lipid modification and biodiesel and chiral organic compound synthesis. sn-1,3-Position-specific Rhizopus lipases are particularly useful for the production of structured triacylglycerols. Significant progress has been made regarding lipases from the genus Rhizopus, including gene sequencing, elucidation of the protein structure and catalytic function, heterologous expression and redesigning Rhizopus lipases for valuable properties, which is receiving increasing academic and industrial attention. In this review, we present a comprehensive overview of Rhizopus lipases, focusing on (a) the characteristics of Rhizopus lipases, (b) Rhizopus lipase genes and structural features, (c) strategies for heterologous expression of Rhizopus lipase genes in yeast system, (d) progress in protein engineering for the improvement of the properties of Rhizopus lipases, and (e) development of biotechnological applications.