Spontaneous Ca²⁺ transients in mouse microglia

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• 作者：Laura Korvers ; Amanda de Andrade Costa ; Martin Mersch ; Vitali Matyash ; Helmut Kettenmann¹ ; Marcus Semtner¹ ; ^{marcus.semtner@mdc-berlin.de}
• 关键词：ATP ; adenosine triphosphate ; Ca2+ ; calcium ions ; [Ca2+]I ; intracellular calcium concentration ; CPA ; cyclopiazonic acid ; ER ; endoplasmic reticulum ; GAM ; glioma-associated microglia ; hGAM ; human GAM ; IP3 ; inositol-1 ; 4 ; 5-trisphosphate ; IP3R ; IP3 receptor ; LPA ; lysophosphatic acid ; LPS ; lipopolysaccharide ; mGAM ; mouse GAM ; P2XR ; P2X receptor ; P2YR ; P2Y receptor ; PLC ; phospholipase C ; PMCA ; plasma membrane Ca2+ ATPase ; RyR ; ryanodine receptor ; SERCA ; sarco-/endoplasmatic Ca2+ ATPase ; UDP ; uridine dip
• 刊名：Cell Calcium
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：60
• 期：6
• 页码：396-406
• 全文大小：2113 K
• 卷排序：60

文摘

Freshly-isolated and cultured microglia display spontaneous and transient cytosolic Ca2+ elevations. Since there were no other cell types these preparations, spontaneous Ca2+ transients most likely occur due to intrinsic microglial signaling. Ca2+ transients originate from the endoplasmic reticulum and are dependent on the activity of phosphlipase C. Auto-/paracrine ATP release is not the trigger for spontaneous Ca2+ transients in microglia. Frequency and kinetics of Ca2+ transients are altered after prolonged LPS stimulation and in the context of glioma.