A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals

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• 作者：Brenez ; Cé ; cile ; Gerkens ; Pascal ; Mazzucchelli ; Gabriel ; Jauniaux ; Thierry ; Eppe ; Gauthier ; et. al.
• 关键词：PCDD/F mixture ; Seal PBMC ; Lymphoproliferation ; Proteomics
• 刊名：Talanta
• 出版年：2004
• 出版时间：August 8, 2004
• 年：2004
• 卷：63
• 期：5
• 页码：1225-1230
• 全文大小：247 K

文摘

The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes.