Role of Kupffer cells and toll-like receptor 4 in acetaminophen-induced acute liver failure

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• 作者：James E. Fisher ; MD^a ; Travis J. McKenzie ; MD^a ; Joseph B. Lillegard ; MD ; PhD^a ; Yue Yu ; MD ; PhD^a ; ^b ; Justin E. Juskewitch^c ; Geir I. Nedredal ; MD ; PhD^a ; Gregory J. Brunn ; PhD^d ; Eunhee S. Yi ; MD^c ; Harmeet Malhi ; MBBS^e ; Thomas C. Smyrk ; MD^c ; Scott L. Nyberg ; MD ; PhD^a ; ^{nyberg.scott@mayo.edu}
• 关键词：E5564 ; Acute liver failure ; Systemic inflammatory response syndrome ; Mice
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：180
• 期：1
• 页码：147-155
• 全文大小：1048 K

文摘

Background

Significant morbidity associated with acute liver failure (ALF) is from the systemic inflammatory response syndrome (SIRS). Toll-like receptor 4 (TLR4) has been shown to play an integral role in the modulation of SIRS. However, little is known about the mechanistic role of TLR4 in ALF. Also, no cell type has been identified as the key mediator of the TLR4 pathway in ALF. This study examines the role of TLR4 and Kupffer cells (KCs) in the development of the SIRS following acetaminophen (APAP)-induced ALF.

Materials and methods

Five groups of mice were established: untreated wild-type, E5564-treated (a TLR4 antagonist), gadolinium chloride -treated (KC-depleted), clodronate-treated (KC-depleted), and TLR4-mutant. Following APAP administration, 72-h survival, biochemical and histologic liver injury, extent of lung injury and edema, and proinflammatory gene expression were studied. Additionally, TLR4 expression was determined in livers of wild-type and KC-depleted mice.

Results

Following APAP administration, wild-type, TLR4-mutant, E5564-treated, and KC-depleted mice had significant liver injury. However, wild-type mice had markedly worse survival compared with the other four treatment groups. TLR4-mutant, E5564-treated, and KC-depleted mice had less lung inflammation and edema than wild-type mice. Selected proinflammatory gene expression (interleukin 1¦Â, interleukin 6, tumor necrosis factor) in TLR4-mutant, E5564-treated, and KC-depleted mice was significantly lower compared with wild-type mice after acute liver injury.

Conclusion

This study demonstrates that survival in APAP-induced ALF potentially correlates with the level of proinflammatory gene expression. This study points to a link between TLR4 and KCs in the APAP model of ALF and, more importantly, demonstrates benefits of TLR4 antagonism in ALF.