Ctf4p facilitates Mcm10p to promote DNA replication in budding yeast

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• 作者：Jiafeng Wang ; Rentian Wu ; Yongjun Lu ; Chun Liang
• 关键词：Ctf4p ; DNA replication ; Mcm10p ; Pol α ; Yeast ; Replication-initiation proteins
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：7 May 2010
• 年：2010
• 卷：395
• 期：3
• 页码：336-341
• 全文大小：626 K

文摘

Ctf4p (chromosome transmission fidelity) has been reported to function in DNA metabolism and sister chromatid cohesion in Saccharomyces cerevisiae. In this study, a ctf4^S143F mutant was isolated from a yeast genetic screen to identify replication-initiation proteins. The ctf4^S143F mutant exhibits plasmid maintenance defects which can be suppressed by the addition of multiple origins to the plasmid, like other known replication-initiation mutants. We show that both ctf4^S143F and ctf4Δ strains have defects in S phase entry and S phase progression at the restrictive temperature of 38 °C. Ctf4p localizes in the nucleus throughout the cell cycle but only starts to bind chromatin at the G1/S transition and then disassociates from chromatin after DNA replication. Furthermore, Ctf4p interacts with Mcm10p physically and genetically, and the chromatin association of Ctf4p depends on Mcm10p. Finally, deletion of CTF4 destabilizes Mcm10p and Pol α in both mcm10-1 and MCM10 cells. These data indicate that Ctf4p facilitates Mcm10p to promote the DNA replication.