Antrodia salmonea induces G₂ cell-cycle arrest in human triple-negative breast cancer (MDA-MB-231) cells and suppresses tumor growth in athymic nude mice

详细信息    查看全文

• 作者：Chia-Ting Chang^a ; ¹ ; You-Cheng Hseu^b ; ^c ; ¹ ; Varadharajan Thiyagarajan^b ; Hui-Chi Huang^d ; Li-Sung Hsu^e ; Pei-Jane Huang^c ; Jer-Yuh Liu^f ; Jiunn-Wang Liao^g ; Hsin-Ling Yang^a ; ^{hlyang@mail.cmu.edu.tw}
• 关键词：Antrodia salmonea ; Breast cancer ; MDA-MB-231cells ; G2 cell-cycle arrest ; Apoptosis ; Autophagy
• 刊名：Journal of Ethnopharmacology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：196
• 期：Complete
• 页码：9-19
• 全文大小：1453 K
• 卷排序：196

文摘

Antrodia salmonea (AS), is a well-known folk medicinal mushroom in Taiwan, has been reported to exhibit anti-oxidant, anti-angiogenic, and anti-inflammatory effects.Materials and methodsIn the present study, we examined the effects of AS on cell-cycle arrest in vitro in MDA-MB-231 cells and on tumor regression in vivo using an athymic nude mice model.ResultsAS (0–200 μg/mL) treatment significantly induced G2 cell-cycle arrest in MDA-MB-231 cells by reducing the levels of cyclin B1, cyclin A, cyclin E, and CDC2 proteins. In addition, N-acetylcysteine (NAC) pretreatment prevented AS induced G2 cell-cycle arrest, indicating that ROS accumulation and subsequent cell cycle arrest might be a major mechanism of AS-induced cytotoxicity. Further, AS treatment decreased COX-2 expression and induced PARP cleavage was significantly reversed by NAC pretreatment in MDA-MB-231 cells. The in vivo study results revealed that AS treatment was effective in terms of delaying the tumor incidence and reducing the tumor growth in MDA-MB-231-xenografted nude mice. TUNEL assay, immunohistochemical staining and Western blotting confirmed that AS significantly modulated the xenografted tumor progression as demonstrated by induction of apoptosis, autophagy, and cell-cycle arrest.ConclusionOur data strongly suggest that Antrodia salmonea could be an anti-cancer agent for human breast cancer.