MDC1-AS, an antisense long noncoding RNA, regulates cell proliferation of glioma

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• 作者：Hongsheng Yue^a ; Jie Zhu^a ; Shugang Xie^b ; Fangfang Li^c ; Qun Xu^d ; ^{qunxu199@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Glioma ; Long non-coding RNA MDC1-AS ; MDC1 ; Cell proliferation
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：81
• 期：Complete
• 页码：203-209
• 全文大小：1751 K

文摘

Growing number of long noncoding RNAs (lncRNAs) are emerging as new modulators in cancer origination and progression. A lncRNA, mediator of DNA damage checkpoint protein 1antisense RNA (MDC1-AS), with unknown function, is the antisense transcript of tumor suppressor MDC1.

Method

In this study, we investigated the expression pattern and functional role of lncRNA MDC1-AS in glioma by using real time PCR and gain-/loss-of-function studies.

Result

5">The results showed that the expression levels of lncRNA MDC1-AS and MDC1 were significantly downregulated in glioma tissues compared with normal brain tissues, and in glioma cell lines U87MG, U251 and HEB. Overexpression of MDC1-AS resulted in significant inhibition of cell proliferation and cell cycle in U87MG and U251. We also found that MDC1-AS expression was positively correlated with MDC1 expression. In addition, the inhibitory role of MDC1-AS was remarkably diminished when MDC1 was knockdown.

Conclusion

Together, the results suggest that MDC1-AS is a novel tumor suppressor through up-regulation of its antisense tumor-suppressing gene MDC1 in glioma and leads us to propose that MDC1-AS may serve as a potential biomarker and therapeutic target for glioma.