Casuarinin suppresses TARC/CCL17 and MDC/CCL22 production via blockade of NF-¦ÊB and STAT1 activation in HaCaT cells

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• 作者：Dong-Joo Kwon^a ; Young-Soo Bae^b ; Sung Mi Ju^a ; Ah Ra Goh^a ; Gi Soo Youn^a ; Soo Young Choi^a ; Jinseu Park^a ; ^{jinpark@hallym.ac.kr}
• 关键词：TARC ; thymus and activation-regulated chemokine ; MDC ; macrophage-derived chemokine ; TNF-¦Á ; tumor necrosis factor-¦Á ; IFN-¦Ã ; interferon-¦Ã
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：27 January 2012
• 年：2012
• 卷：417
• 期：4
• 页码：1254-1259
• 全文大小：716 K

文摘

Casuarinin is a naturally occurring tannin that is isolated from the leaves of Hippophae rhamnoides. It has been shown to have anti-oxidant, anti-cancer, anti-viral, and anti-inflammatory activities. The aim of this study was to investigate the possible mechanism by which casuarinin inhibits TNF-¦Á/IFN-¦Ã-induced Th2 chemokines expression in the human keratinocytes cell line HaCaT. We found that casuarinin suppressed TNF-¦Á/IFN-¦Ã-induced expression of TARC and MDC mRNA and protein in HaCaT cells. Casuarinin significantly inhibited TNF-¦Á/IFN-¦Ã-induced activation of NF-¦ÊB, STAT1, and p38 MAPK. Furthermore, we observed that p38 MAPK contributes to inhibition of TNF-¦Á/IFN-¦Ã-induced TARC and MDC production by blocking NF-¦ÊB and STAT1 activation in HaCaT cells. Taken together, these results suggest that casuarinin may exert anti-inflammatory responses by suppressing TNF-¦Á/IFN-¦Ã-induced expression of TARC and MDC via blockage of p38 MAPK activation and subsequent activation of NF-¦ÊB and STAT1. We propose that it could therefore be used as a therapeutic agent against inflammatory skin diseases.