Evaluation and validation of reference gene stability during Marek’s disease virus (MDV) infection
详细信息 查看全文
- 作者：Sabari Nath Neerukondaa ; Nnvsnath@udel.edu" class="auth_mail" title="E-mail the corresponding author ; Upendra K. Katnenia ; Upendra@udel.edu" class="auth_mail" title="E-mail the corresponding author ; Sergey Golovanb ; Sergey.Golovan@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Mark S. Parcellsa ; Parcells@udel.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Reference gene ; qRT-PCR ; Data normalization ; Marek&rsquo ; s disease virus ; Lymphomas
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：111-116
- 全文大小：655 K
文摘
- •
We examined the stability of (5) common housekeeping genes during Marek’s disease virus (MDV) infection: beta actin (BACT), 18 S RNA, 28 S RNA, GAPDH, and peptidyl prolyl-isomerase B (PPIB).
- •
Samples examined were from vaccine- and oncogenic-MDV-infected chicken embryo fibroblasts and MDV-induced lymphomas.
- •
Stability was assessed using Bestkeeper and Normfinder algorithms.
- •
Both Bestkeeper and Normfinder algorithms determined that 28 S RNA and PPIB were the most stably expressed during all MDV infection models and the BACT was the least stable.
- •
Our data suggest that 28 S and PPIB should be used for normalization of qRT-PCR expression during MDV infection studies.