Dynamically tunable cell culture platforms for tissue engineering and mechanobiology

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• 作者：Koichiro Uto^a ; Jonathan H. Tsui^a ; Cole A. DeForest^a ; ^b ; ^{profcole@uw.edu} ; Deok-Ho Kim^a ; ^{deokho@uw.edu}
• 关键词：Dynamic cell culture ; Stimuli-responsive material ; Hydrogels ; Mechanobiology ; 4D biology ; Tissue engineering
• 刊名：Progress in Polymer Science
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：65
• 期：Complete
• 页码：53-82
• 全文大小：6904 K
• 卷排序：65

文摘

Human tissues are sophisticated ensembles of many distinct cell types embedded in the complex, but well-defined, structures of the extracellular matrix (ECM). Dynamic biochemical, physicochemical, and mechano-structural changes in the ECM define and regulate tissue-specific cell behaviors. To recapitulate this complex environment in vitro, dynamic polymer-based biomaterials have emerged as powerful tools to probe and direct active changes in cell function. The rapid evolution of polymerization chemistries, structural modulation, and processing technologies, as well as the incorporation of stimuli-responsiveness, now permit synthetic microenvironments to capture much of the dynamic complexity of native tissue. These platforms are comprised not only of natural polymers chemically and molecularly similar to ECM, but those fully synthetic in origin. Here, we review recent in vitro efforts to mimic the dynamic microenvironment comprising native tissue ECM from the viewpoint of material design. We also discuss how these dynamic polymer-based biomaterials are being used in fundamental cell mechanobiology studies, as well as toward efforts in tissue engineering and regenerative medicine.