Real-time quantitative PCR assay with Taqman^® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance

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• 作者：S. Chabou ; T. Leangapichart ; L. Okdah ; S. Le Page ; L. Hadjadj ; J.-M. Rolain ; ^{jean-marc.rolain@univ-amu.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antibiotic resistance surveillance ; colistin resistance ; mcr-1 gene ; PCR detection ; Taqman probe
• 刊名：New Microbes and New Infections
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：13
• 期：Complete
• 页码：71-74
• 全文大小：271 K

文摘

Here we report the development of two rapid real-time quantitative PCR assays with TaqMan^® probes to detect the MCR-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. Specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six Klebsiella pneumoniae and 12 Escherichia coli) with a calibration curve that was linear from 10¹ to 10⁸ DNA copies. Five out of 833 faecal samples from chickens from Algeria were positive, from which three E. coli strains were isolated and confirmed to harbour the mcr-1 gene by standard PCR and sequencing.