Ionic liquid (IL) and organosolv (OV) pretreatments were applied on AGB. Sequential saccharification and fermentation (SESF) was used on pretreated AGB. High lignin and xylan removal were achieved with IL and OV pretreatments. Total sugars consumption with no solids removal occurred during SESF. Based on 100 kg raw AGB, ethanol yield was 12.1 kg for IL and 12.7 kg for OV.