Self-assembled luminescent CdSe–ZnS quantum dot bioconjugates prepared using engineered poly-histidine terminated proteins
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- 作者：Goldman ; Ellen R. ; Medintz ; Igor L. ; Hayhurst ; Andrew ; Anderson ; George P. ; Mauro ; J. Matthew ; et. al.
- 关键词：Quantum dot ; Histidine tag ; Affinity tag ; Bioconjugation ; Single chain antibody ; scFv ; TNT ; Immunoassay
- 刊名：Analytica Chimica Acta
- 出版年：2005
- 出版时间：April 4, 2005
- 年：2005
- 卷：534
- 期：1
- 页码：63-67
- 全文大小：75.6 K
文摘
We report a simple and versatile approach for the conjugation of luminescent CdSe–ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences. Several histidine tail containing proteins were self-assembled onto the QD surface using this method. A recombinant antibody specific for the high explosive 2,4,6-trinitrotoluene (TNT) was conjugated to QDs through a carboxy terminal histidine tail and the bioconjugate used to detect TNT by competitive immunoassay. TNT was detected over the range of 10 μg/ml down to 41 ng/ml using the scFv conjugated to QDs. These results open up the possibility to conjugate luminescent QDs to a whole range of proteins to form QD bioconjugates that can be effectively used in bio-oriented applications, such as sensing, imaging, immunoassay and other diagnostics.