Electrophoretic karyotyping and construction of a bacterial artificial chromosome library of the winter mushroom Flammulina velutipes

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• 作者：Young-Jin Park ; Jong-Kun Kim ; Won-Sik Kong ; Eun-Sung Song ; Chang-soo Lee ; Hyungtae Kim ; Jang-Ho Hahn ; Hee-Wan Kang ; Byoung-Moo Lee
• 关键词：Flammulina velutipes ; Electrophoretic karyotyping ; Bacterial artificial chromosome (BAC) ; Contour-clamped homogeneous electric field (CHEF)
• 刊名：Microbiological Research
• 出版年：2010
• 出版时间：30 May 2010
• 年：2010
• 卷：165
• 期：4
• 页码：321-328
• 全文大小：360 K

文摘

Summary

An electrophoretic karyotype of Korean Flammulina velutipes was obtained using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The monokaryotic progenies (4019-20 and 4019-18) and a dikaryotic strain (4019-2018) had 6–8 chromosomes, with sizes ranging from 1.6 to 5.8 megabase (Mb) pairs. Among the 3 strains that were examined, strain 4019-20 resolved into at least 7 chromosomes, with a total genome size of approximately 26.7 Mb. We selected several chromosome-specific genes from the cDNA library of F. velutipes using Southern hybridization analysis. In order to determine the whole genome sequence, we constructed a bacterial artificial chromosome (BAC) library of the 4019-20 strain. The BAC library comprised 3840 clones, and the insert size ranged from 60 to 228 kb, with an average size of 136 kb.