Fibrin matrices: The versatile therapeutic delivery systems
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- 作者：Ejaj Ahmad ; Munazza Tamkeen Fatima ; Mehboob Hoque ; Mohammad Owais ; Mohammed Saleemuddin ; msaleemuddin@rediffmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：AdeNOS ; adeno viral vector encoding endothelial nitric oxide synthase ; ADSCs ; adipose-derived stem cells ; ALP ; alkaline phosphatase ; AT-COL ; atelocollagen ; bFGF ; basic fibroblast growth factor ; BMMNCs ; bone marrow mononuclear cells ; BMMSCs ; bone marrow derived mesenchymal stem cells ; BMP-2 ; bone morphogenetic protein-2 ; BMSC ; bone marrow stem cells ; CEG ; culture of epidermal sheets graft ; COL1A1 ; collagen type 1 alpha 1 ; Cp ; candida cytosolic proteins ; DRGs ; dorsal root ganglia ; EDC ; 1-ethyl-3-(
- 刊名：International Journal of Biological Macromolecules
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：81
- 期：Complete
- 页码：121-136
- 全文大小：2449 K
文摘
Fibrin sealants, that have been employed for over a century by surgeons to stop post surgery bleeding, are finding novel applications in the controlled delivery of antibiotics and several other therapeutics. Fibrinogen can be easily purified from blood plasma and converted by thrombolysis to fibrin that undergoes spontaneous aggregation to form insoluble clot. During the gelling, fibrin can be formulated into films, clots, threads, microbeads, nanoconstructs and nanoparticles. Whole plasma clots in the form of beads and microparticles can also be prepared by activating endogenous thrombin, for possible drug delivery. Fibrin formulations offer remarkable scope for controlling the porosity as well as in vivo degradability and hence the release of the associated therapeutics. Binding/covalent-linking of therapeutics to the fibrin matrix, crosslinking of the matrix with bifunctional reagents and coentrapment of protease inhibitors have been successful in regulating both in vitro and in vivo release of the therapeutics. The release rates can also be remarkably lowered by preentrapment of therapeutics in insoluble particles like liposomes or by anchoring them to the matrix via molecules that bind them as well as fibrin.