Fc-specific biotinylation of antibody using an engineered photoactivatable Z-Biotin and its biosensing application
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- 作者：Hong-Ming Yang ; Ru-Meng Bao ; Chang-Mei Yu ; Yan-Na Lv ; Wei-Fen Zhang ; Jin-Bao Tang ; tangjb@wfmc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; tangjinbao2002@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Photoactivatable Z&ndash ; Biotin ; Fc-specific covalent conjugation ; Immunoglobulin G ; Biotin ; Biosensing
- 刊名：Analytica Chimica Acta
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：949
- 期：Complete
- 页码：76-82
- 全文大小：1453 K
文摘
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A photoactivable ZBpa–Biotin was fabricated by aaRS/tRNA and Avitag/BirA techniques.
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A approach for Fc-specific photo-biotinylated IgG via ZBpa–Biotin was proposed.
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The photo-biotinylated IgG was used to fabricate an immunosensor for detecting CEA.
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It gave a LOD of 2 ng mL-1 CEA, was 5-fold lower than that of NHS-biotinylated IgG.