- 作者：Yong Yuan ; Bo Yang ; Zuowu Ye ; Meiling Zhang ; Xiuli Yang ; Chuanwei Xin ; Mengmeng Lin ; Ping Huang
- 关键词：Sceptridium ternatum Lyon (ST) ; Traditional chinese medicine ; Immunomodulation ; Th1/Th2 ; Airway responsiveness ; Asthma
- 刊名：Journal of Ethnopharmacology
- 出版年：2013
- 出版时间：7 October, 2013
- 年：2013
- 卷：149
- 期：3
- 页码：701-706
- 全文大小：1529 K
Aim of the study
Sceptridium ternatum Lyon (ST), a common Chinese herb, has been used in treatment of allergic asthma and whooping cough. In the present study, we investigated the Th1/Th2 ratio of peripheral blood and mRNA levels of leukotriene receptors after the treatment of ST in allergic asthma mouse model.Materials and methods
Mouse asthma model was developed by ovalbumin (OVA) sensitization followed by the inhalation of aerosol allergen. Montelukast (10 mg/kg), as a positive control drug, and ST were administrated six days before the OVA sensitization for ten days. Airway responsiveness was evaluated by the Medlab 12.0 biological signal processing system. The ratio of Th1/Th2 cells was determined by flow cytometry. The expression level of Cyslt1 was measured by PCR. Pathological changes of lung tissues were examined by H&E staining.
Results
ST significantly reduced the airway responsiveness, elevated the ratio of Th1/Th2, and decreased Cyslt1 mRNA level in a dose-dependent manner. High-dose ST distinctly prevented the pathological changes of lung tissues.
Conclusion
High-dose ST had the same efficacy as Montelukast in a mouse asthma model, and ST could be a potential anti-asthmatic agent.