Protamine 2 precursors (Pre-P2), protamine 1 to protamine 2 ratio (P1/P2), and assisted reproduction outcome

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• 作者：Sara de Mateo ; Cristina Gá ; zquez ; Marta Guimerà ; Juan Balasch ; Marvin L. Meistrich ; José ; Luis Ballescà ; Rafael Oliva
• 关键词：Spermatozoa ; assisted reproduction outcome ; protamine ; protamine 2 precursors ; pre-P2 ; IVF ; ICSI ; sperm ; nucleus
• 刊名：Fertility and Sterility
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：91
• 期：3
• 页码：715-722
• 全文大小：226 K

文摘

lass=""h4"">Objective

To determine whether the presence of protamine 2 precursors (pre-P2/P2 ratio) and the protamine 1 to protamine 2 ratio (P1/P2) are related to the assisted reproduction outcome.

lass=""h4"">Design

Prospective study.

lass=""h4"">Setting

Assisted Reproduction Unit and University laboratory.

lass=""h4"">Patient(s)

One hundred two infertile patients undergoing treatment at the Assisted Reproduction Unit of the Hospital Clinic of Barcelona.

lass=""h4"">Intervention(s)

Intracytoplasmic sperm injection (ICSI) and/or IVF treatment of the infertile patients, sperm protamine analysis through electrophoresis and densitometry, and pre-P2 analysis through Western blot.

lass=""h4"">Main Outcome Measure(s)

The presence of protamine 2 precursors (pre-P2/P2 ratio), sperm P1/P2 ratio, fertilization rates by IVF and/or ICSI, and pregnancy outcome.

lass=""h4"">Result(s)

Pre-P2/P2 and P1/P2 ratios are positively associated with the pregnancy rate. In addition, the P1/P2 ratio is positively associated with the proportion of embryos obtained by IVF, but not by ICSI. The pre-P2/P2 ratio was not related to the fertilization rate.

lass=""h4"">Conclusion(s)

Decreased pre-P2/P2 and P1/P2 ratios are related to a poor pregnancy outcome, but not with the proportion of embryos obtained after ISCI.