Protamine 2 precursors (Pre-P2), protamine 1 to protamine 2 ratio (P1/P2), and assisted reproduction outcome

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• 作者：Sara de Mateo ; Cristina Gá ; zquez ; Marta Guimerà ; Juan Balasch ; Marvin L. Meistrich ; José ; Luis Ballescà ; Rafael Oliva
• 关键词：Spermatozoa ; assisted reproduction outcome ; protamine ; protamine 2 precursors ; pre-P2 ; IVF ; ICSI ; sperm ; nucleus
• 刊名：Fertility and Sterility
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：91
• 期：3
• 页码：715-722
• 全文大小：226 K

文摘

Objective

To determine whether the presence of protamine 2 precursors (pre-P2/P2 ratio) and the protamine 1 to protamine 2 ratio (P1/P2) are related to the assisted reproduction outcome.

Design

Prospective study.

Setting

Assisted Reproduction Unit and University laboratory.

Patient(s)

One hundred two infertile patients undergoing treatment at the Assisted Reproduction Unit of the Hospital Clinic of Barcelona.

Intervention(s)

Intracytoplasmic sperm injection (ICSI) and/or IVF treatment of the infertile patients, sperm protamine analysis through electrophoresis and densitometry, and pre-P2 analysis through Western blot.

Main Outcome Measure(s)

The presence of protamine 2 precursors (pre-P2/P2 ratio), sperm P1/P2 ratio, fertilization rates by IVF and/or ICSI, and pregnancy outcome.

Result(s)

Pre-P2/P2 and P1/P2 ratios are positively associated with the pregnancy rate. In addition, the P1/P2 ratio is positively associated with the proportion of embryos obtained by IVF, but not by ICSI. The pre-P2/P2 ratio was not related to the fertilization rate.

Conclusion(s)

Decreased pre-P2/P2 and P1/P2 ratios are related to a poor pregnancy outcome, but not with the proportion of embryos obtained after ISCI.