3-Iodothyronamine increases transient receptor potential melastatin channel 8 (TRPM8) activity in immortalized human corneal epithelial cells
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- 作者：Alexander Luciusa ; Noushafarin Khajavia ; Peter S. Reinachb ; Josef Kö ; hrlec ; Priyavathi Dhandapanid ; Philipp Huimanna ; Nina Ljubojevica ; Carsten Grö ; tzingerd ; Stefan Merglera ; stefan.mergler@charite.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：HCEC ; human corneal epithelial cells ; DED ; dry eye disease ; TAM ; thyronamine ; 3T1AM ; = ; 3-iodothyronamine ; TRPV ; transient receptor potential vanilloid ; TRPM ; transient receptor potential melastatin ; CAP ; capsaicin ; BCTC ; N-(4-tertiarybutyl-phenyl)-4-(3-chloropyridin-2-yl) tetrahydropyrazine-1(2H)-carboxamide ; La3 ; + ; ; lanthanum-III-chloride ; AMTB ; N-(3-aminopropyl)-2-[(3-methylphenyl)methoxy]-N-(2-thienylmethyl)benzamide hydrochloride
- 刊名：Cellular Signalling
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：28
- 期：3
- 页码：136-147
- 全文大小：2237 K
文摘
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T1AM is an endogenous thyroid hormone (TH) metabolite inducing a reversible body temperature decline.
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Transient receptor potential melastatin 8 (TRPM8) is expressed in human corneal epithelial cells.
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We studied TRPM8 and T1AM in regulating [Ca2 +]i and TRPV1 interaction in HCEC.
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PCR, immunohistochemistry, Ca2 + imaging, planar patch-clamp technique, and heterologous TRPM8 expressed cells
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TRPM8 activation suppressed TRPV1 activation by either capsaicin or exposure to a 450 mOsm hypertonic stress.