Construction and phenotypic characterization of M68, an RruI quorum sensing knockout mutant of the photosynthetic alphaproteobacterium Rhodospirillum rubrum

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• 作者：Sandra Condori^a ; ^b ; ^{sccatach@sckcen.be" class="auth_mail" title="E-mail the corresponding author} ; Steve Atkinson^c ; ^{Steve.Atkinson@nottingham.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Natalie Leys^a ; ^{nleys@sckcen.be" class="auth_mail" title="E-mail the corresponding author} ; Ruddy Wattiez^b ; ^{ruddy.wattiez@umons.ac.be" class="auth_mail" title="E-mail the corresponding author} ; Felice Mastroleo^a ; ^{fmastrol@sckcen.be" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Quorum sensing ; Pigments ; Biofilm ; Low shear environment ; MELiSSA ; HdtS
• 刊名：Research in Microbiology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：167
• 期：5
• 页码：380-392
• 全文大小：1737 K

文摘

Many bacterial species communicate using a complex system known as quorum sensing (QS) in which gene expression is controlled in response to cell density. In this study an N-acylhomoserine lactone (AHL) synthase (Rru_A3396) knockout mutant (M68) of Rhodospirillum rubrum S1H (WT) was constructed and characterized phenotypically under light anaerobic conditions. Results showed that R. rubrum WT produces unsubstituted, 3-OH and 3-oxo-substituted AHLs with acyl chains ranging from 4 to 14 carbons, with 3-OH-C8 being the most abundant. Growth, pigment content and swimming motility were found to be under the control of this LuxI-type QS system. In addition, cultivation in a low shear environment put forward the aggregative phenotype of M68 and linked biofilm formation to QS in R. rubrum S1H. Interestingly, QS-mutant M68 continued to produce decreased levels of 3-OH-C8-HSL, probably due to the presence of an extra HdtS-type AHL synthase.