Quantification of Pea enation mosaic virus 1 and 2 during infection of Pisum sativum by one step real-time RT-PCR

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• 作者：Juliette Doumayrou^a ; ¹ ; ^{juliette.doumayrou@gmail.com} ; Melissa Sheber^a ; Bryony C. Bonning^b ; W. Allen Miller^a ;
• 关键词：Pea enation mosaic virus ; Pisum sativum ; Normalized quantification ; Virus accumulation
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：63-68
• 全文大小：575 K
• 卷排序：240

文摘

Normalized quantitative real-time one-step reverse transcription PCR method to assess PEMV1 and 2 replication in pea plants with two inoculum conditions. Compared two different housekeeping genes for normalization of viral accumulation data. Accumulation of both viral RNAs was similar whether actin or β-tubulin mRNAs were used as reference transcripts. PEMV1 replication dynamics were changed by inoculum concentration when compared to PEMV2.