Development and monitoring of an integrated bioprocess for production of a potential malaria vaccine with Pichia pastoris
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- 作者：Sven-Oliver Borchert ; Tobias Voss ; Fabian Schuetzmeier ; Jessica Paul ; Gesine Cornelissen ; Reiner Luttmann ; reiner.luttmann@haw-hamburg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：AMA1 ; apical membrane antigen 1 ; BMBF ; German Federal Ministry of Education and Research ; BPRC ; Biomedical Primate Research Center ; BWF ; Hamburg Ministry of Science and Research ; CCC ; central composite circumscribed ; CV ; column volume ; D1M1 ; fusion protein composed of AMA1 and MSP1 ; DModX ; distance to model ; DoE ; design of experiments ; EBA ; expanded bed adsorption ; FDA ; Food and Drug Administration ; FF ; fast flow ; FPLC ; fast protein liquid chromatography ; gb ; golden batch ; HPLC ; high performance
- 刊名：Journal of Process Control
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：35
- 期：Complete
- 页码：113-126
- 全文大小：5175 K
文摘
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Pichia pastoris was used for expression of a potential malaria vaccine.
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10">An integrated bioprocess with expanded bed adsorption was set up.
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15">Design of experiments was applied successfully for process optimization.
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A golden batch model for monitoring of the integrated process was constructed.