Heterogeneous MAC Initiator and Pore Structures in a Lipid Bilayer by Phase-Plate Cryo-electron Tomography
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- 作者：Thomas H. Sharp1 ; t.sharp@lumc.nl" class="auth_mail" title="E-mail the corresponding author ; Abraham J. Koster1 ; 2 ; Piet Gros3 ; p.gros@uu.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：phase plate ; membrane attack complex ; complement ; electron tomography ; subtomogram average ; membrane pore
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：5 April 2016
- 年：2016
- 卷：15
- 期：1
- 页码：1-8
- 全文大小：3520 K
文摘
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MAC pores are cone shaped, twisted, and poorly sealed between C9 end and C5b-8 start
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Complement activation yields single and joined MAC pores in lipid membranes
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Oligomerization of three to four C5b-8 complexes causes ∼3.5-nm pores in membranes
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Phase plates markedly increase interpretability of cryo-electron tomograms