We expressed the recombinant proteins of the following OmpA fragments by using molecular cloning of Loop 1–2, Loop 1–3, Loop 1–4, Loop 2–3, Loop 2–4, and Loop 3–4. Animal experiments were subsequently performed to investigate the effects of these recombinant OmpA fragments on the survival of C57BL/6 mice after intracerebral E. coli RS218 administration.
This study demonstrated that the recombinant Loop 1–3, Loop 2–3, and Loop 2–4 fragments of OmpA can protect mice from intracerebral E. coli infection.
In bacterial meningitis, although antibiotic therapy is the first choice for management, neurological complications can seldom be averted. Based on the results of the present study, we intend to establish an effective therapeutic application for E. coli meningitis.