Selective excitation for spectral editing and assignment in separated local field experiments of oriented membrane proteins

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• 作者：Sophie N. Koroloff ; Alexander A. Nevzorov ; ^{alex_nevzorov@ncsu.edu}
• 关键词：Selective excitation ; Soft Gaussian pulses ; Separated local field experiments ; SAMPI4 ; Spectral editing ; Oriented samples ; Membrane proteins ; Solid-state NMR
• 刊名：Journal of Magnetic Resonance
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：274
• 期：Complete
• 页码：7-12
• 全文大小：1253 K
• 卷排序：274

文摘

Spin-exchanged separated local field spectra exhibit many overlapping resonances. Selective Gaussian pulses are employed to excite resolved ‘seed’ resonances. Polarization is then transferred to their nearest neighbors to initiate assignment. Cross peaks are evolved at the locations of main peaks, thus simplifying the spectrum. A single, uniformly labeled sample is used.