Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

详细信息    查看全文

• 作者：Dongsheng Guo^a ; ^b ; Feima Wu^a ; ^b ; Haikun Liu^a ; ^b ; Ge Gao^a ; ^b ; Shanglong Kou^a ; ^b ; Fan Yang^a ; ^b ; Nasir Abbas^a ; ^b ; Tiancheng Zhou^b ; Xiujuan Cai^b ; Hui Zhang^b ; Dajiang Qin^b ; Jialiang Li^d ; Kecheng Xu^d ; ^{xukc@vip.163.com} ; Yin-xiong Li^a ; ^b ; ^c ; ^{li_yinxiong@gibh.ac.cn}
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：64-66
• 全文大小：532 K
• 卷排序：18

文摘

Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G > T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.