Characterisation of separated end hyaluronan oligosaccharides from leech hyaluronidase and evaluation of angiogenesis
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- 作者：Mengxian Lva ; Miao Wanga ; mwang423@126.com" class="auth_mail" title="E-mail the corresponding author ; mwang@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Weiwei Caib ; Wenxing Haoa ; Panhong Yuanc ; Zhen Kangc
- 关键词：Leech hyaluronidase ; Hyaluronan tetrasaccharide ; Hyaluronan hexasaccharide ; Quantitative analysis ; Chromatography ; Angiogenesis
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：20 May 2016
- 年：2016
- 卷：142
- 期：Complete
- 页码：309-316
- 全文大小：2085 K
文摘
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0.42 g HA4 and 0.46 g HA6 were obtained from 1 g HA with 16,000 U/mL LHase.
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Accurate control of the proportion of HA4 and HA6 was achieved.
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Highly pure HA4 and HA6 were prepared by ion exchange Q-Sepharose Fast Flow.
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HA4 and HA6 from LHase showed high activity in promotion of angiogenesis.