- 作者：Ji ZHANG ; Hai Jian ZHOU ; Li XU ; Guang Chun HU ; Xue Hua ZHANG ; Sheng Ping XU ; Zun Yu LIU ; Zhu Jun SHAO
- 关键词：Pulsed-field gel electrophoresis ; Multilocus sequence typing ; Neisseria meningitidis ; ST-4821 ; Serogroup C
- 刊名：Biomedical and Environmental Sciences
- 出版年：2013
- 出版时间：May, 2013
- 年：2013
- 卷：26
- 期：5
- 页码：331-337
- 全文大小：1780 K
Objective
To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak.Methods
The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST.
Results
Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type (ST) 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14 % (375/416) serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010.
Conclusion
Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.