Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects

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• 作者：V¨ªctor D. Busto ; Ariel Calabr¨®-L¨®pez ; Juli¨¢n Rodr¨ªguez-Talou ; Ana M. Giulietti ; Jos¨¦ C. Merchuk
• 关键词：Anthraquinones ; Shear stress ; Turbulence ; Scale-down ; Rubia tinctorum ; Plant cell cultures ; Shaken bioreactors
• 刊名：Biochemical Engineering Journal
• 出版年：2013
• 出版时间：15 August, 2013
• 年：2013
• 卷：77
• 期：Complete
• 页码：119-128
• 全文大小：1827 K

文摘

The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008 ). The effect of light on AQs production was found to be very significant, and a difference of up to 48 % was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.