Over-expression of poplar transcription factor ERF76 gene confers salt tolerance in transgenic tobacco

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• 作者：Wenjing Yao^a ; Lei Wang^b ; Boru Zhou^a ; Shengji Wang^a ; Renhua Li^a ; Tingbo Jiang^a ; ^{tbjiang@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：TF ; transcription factor ; ERF ; ethylene response factor ; RWC ; relative water content ; SOD ; superoxide dismutase ; POD ; peroxidase ; MDA ; malondialdehyde ; REC ; relative electrical conductivity ; ROS ; reactive oxygen species ; DAB ; diaminobenzidine ; NBT ; nitrotetrazolium blue chloride ; CL ; control line containing pBI121 ; TL ; transgenic line over-expressing ERF76 gene
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 July 2016
• 年：2016
• 卷：198
• 期：Complete
• 页码：23-31
• 全文大小：3784 K

文摘

Ethylene response factors (ERFs) belong to a large plant-specific transcription factor family, which play a significant role in plant development and stress responses. Poplar ERF76 gene, a member of ERF TF family, can be up-regulated in response to salt stress, osmotic stress, and ABA treatment. The ERF76 protein was confirmed to be targeted preferentially in the nucleus of onion cell by particle bombardment. In order to understand the functions of ERF76 gene in salt stress response, we conducted temporal and spatial expression analysis of ERF76 gene in poplar. Then the ERF76 cDNA fragment containing an ORF was cloned from di-haploid Populus simonii × P. nigra and transferred into tobacco (Nicotiana tobacum) genome by Agrobacterium-mediated leaf disc method. Under salt stress, transgenic tobacco over-expressing ERF76 gene showed a significant increase in seed germination rate, plant height, root length, and fresh weight, as well as in relative water content (RWC), superoxide dismutase (SOD) activity, peroxidase (POD) activity, and proline content, compared to control tobacco lines. In contrast, transgenic tobacco lines displayed a decrease in malondialdehyde (MDA) accumulation, relative electrical conductivity (REC) and reactive oxygen species (ROS) accumulation in response to salt stress, compared to control tobacco lines. Over all, the results indicated that ERF76 gene plays a critical role in salt tolerance in transgenic tobacco.