Regenerative efficacy of mesenchymal stromal cells from human placenta in sensorineural hearing loss

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• 作者：Kicheol Kil^a ; ¹ ; Mi Young Choi^b ; ^c ; ¹ ; Ji Sun Kong^c ; Woo Jin Kim^c ; Kyoung Ho Park^c ; ^{khpent@catholic.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Amnion ; Chorion ; Mesenchymal stromal cell ; Hearing loss
• 刊名：International Journal of Pediatric Otorhinolaryngology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：91
• 期：Complete
• 页码：72-81
• 全文大小：3465 K

文摘

Hearing loss is a common chronic disorder characterized by decline of auditory function. The global population have suffered from deafness and the transplantation of stem cells is regarded as a therapeutic strategy for this disease.

Methods

We collected placenta from a total of 13 samples of full term pregnant women and isolated MSCs derived from human placenta and transplanted MSCs on deaf animal model. The normal group and the sensorineural hearing loss (SNHL) group and the experimental (transplanted MSCs) group were compared and estimated hearing level using auditory brainstem response (ABR) recordings and the otoacoustic emission (OAE) test.

Results

ABR threshold value and DPOAE level showed that MSCs transplantation groups was improved than the SNHL group. And the number of spiral ganglion neurons were increased in all turn of the cochlea. And there was no evidence of acute immunological rejection and inflammation response was not observed.

Discussion

This study is to evaluate regenerative efficacy of hearing loss by transplanting mesenchymal stromal cells (MSCs) derived from human placenta (amnion and chorion) in deaf animal model. We identified that MSCs transplantation restored auditory impairment and promoted cell regeneration. We hope to overcome sensorineural hearing loss by transplanting stem cells such as mesenchymal stromal cells (MSCs) from easily accessible adult stem cell source in placenta.