Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection
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- 作者：Paulina Furmaniak ; Paweł Kubalczyk ; pakuba@uni.lodz.pl" class="auth_mail" title="E-mail the corresponding author ; Justyna Stachniuk ; Rafał Głowacki ; glowar@uni.lodz.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：CMLT ; 2-chloro-l-methyllepidinium tetrafluoroborate ; Mesna ; Sodium 2-mercaptoethanesulfonate ; MPS ; Sodium 3-mercaptopropanesulfonate ; TCEP ; Tris(2-carboxyethyl)phosphine
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 August 2016
- 年：2016
- 卷：1027
- 期：Complete
- 页码：88-95
- 全文大小：920 K
文摘
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Simple, cheap and fast MEKC assay for total mesna in human plasma was elaborated.
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For the first time CMLT as an effective derivatization reagent in CE was used.
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In-capillary derivatization of mesna with CMLT was applied.
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Stacking with high salt concentration in MEKC was successfully employed.
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The method exploits cheap and user friendly UV detection.