Generation and characterization of human iPSC line from CD34⁺ cells isolated from umbilical cord blood belonging to Indian origin

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• 作者：Sophia Fernandes^a ; Manasi Talwadekar^a ; Raju Agarwal^b ; ¹ ; Velu Nair^c ; ² ; Vaijayanti Kale^a ; Lalita Limaye^a ; ^{lslimaye@nccs.res.in}
• 刊名：Stem Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：18
• 期：Complete
• 页码：60-63
• 全文大小：657 K
• 卷排序：18

文摘

We describe here the reprogramming of CD34+ cells isolated from umbilical cord blood obtained after full term delivery of a healthy female child of Indian origin. The cells were nucleofected by episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). Colonies were identified by alkaline phosphatase staining and characterized for expression of pluripotency markers at protein level by immunofluorescence, flow cytometry and at transcript level by PCR. Genomic stability of the cell line was checked by G-banded karyotype. The ability to differentiate to endoderm, mesoderm and ectoderm in vitro was confirmed by immunofluorescence staining.