Structure of the O-polysaccharides of the lipopolysaccharides of Mesorhizobium loti HAMBI 1148 and Mesorhizobium amorphae ATCC 19655 containing two O-methylated monosaccharides

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• 作者：Evelina L. Zdorovenko ; Olga A. Valueva ; Vadim V. Kachala ; Alex ; er S. Shashkov ; Nina A. Kocharova ; Yuriy A. Knirel ; Jolanta Kutkowska ; Anna Turska-Szewczuk ; Teresa Urbanik-Sypniewska ; Adam Choma ; Rysza
• 关键词：Lipopolysaccharide ; Bacterial polysaccharide structure ; O-Methylated monosaccharide ; Mesorhizobium loti ; Mesorhizobium amorphae
• 刊名：Carbohydrate Research
• 出版年：2009
• 出版时间：14 December 2009
• 年：2009
• 卷：344
• 期：18
• 页码：2519-2527
• 全文大小：882 K

文摘

The O-polysaccharide of Mesorhizobium loti HAMBI 1148 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation, and ¹H and ¹³C NMR spectroscopies, including 2D ¹H/¹H COSY, TOCSY, ROESY, and H-detected ¹H/¹³C HSQC experiments. The O-polysaccharide was found to have a branched hexasaccharide-repeating unit of the following structure:

where 2-acetamido-2-deoxy-4-O-methyl-d-glucose (d-GlcNAc4Me) and methyl group on 2-substituted d-rhamnose (Me) shown in italics are present in 80 % and 40 % repeating units, respectively. Similar studies of the O-polysaccharide from Mesorhizobium amorphae ATCC 19655 by sugar analysis and NMR spectroscopy revealed essentially the same structure but a higher content of 3-O-methyl-d-rhamnose (70 % ).