Optimal tracers for parallel labeling experiments and 13C metabolic flux analysis: A new precision and synergy scoring system
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- 作者：Scott B. Crown1 ; Christopher P. Long1 ; Maciek R. Antoniewicz ; mranton@udel.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Optimal tracer experiment design ; Isotopic labeling ; E. coli ; Integrated flux analysis ; GC&ndash ; MS
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：10-18
- 全文大小：1990 K
文摘
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New method is developed for selection of optimal tracers for 13C metabolic flux analysis.
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Doubly 13C-labeled glucose tracers are optimal, and the best tracer is [1,6-13C]glucose.
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Selection of optimal tracers is not strongly influenced by assumed metabolic fluxes.
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[1,6-13C]glucose and [1,2-13C]glucose are optimal tracers for parallel labeling experiments.
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[2,5-13C]glucose and [3,4-13C]glucose are a good tracer to complement other tracers.