Development and validation of an enantioselective SFC-MS/MS method for simultaneous separation and quantification of oxcarbazepine and its chiral metabolites in beagle dog plasma
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- 作者：Zhichao Yanga ; Xueyu Xua ; Lingxia Suna ; Xue Zhaoa ; Hao Wanga ; John Paul Fawcettb ; Yan Yanga ; yyan@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jingkai Guc ; gujk@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：SFC-MS/MS ; UPC2 ; Oxcarbazepine ; Chiral analysis ; S-Licarbazepine ; R-Licarbazepine
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 May 2016
- 年：2016
- 卷：1020
- 期：Complete
- 页码：36-42
- 全文大小：1079 K
文摘
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An enantioselective SFC-MS/MS assay has been validated for the simultaneous separation and quantification of oxcarbazepine and its chiral metabolites in beagle dog plasma at low pg/mL range.
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The resolution of S-Lic and R-Lic was very satisfying by careful optimization of chromatographic conditions and selection of MRM transitions at the run time of only 3 min.
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The validated 3-in-1 method has been applied to a pharmacokinetic study for the simultaneous determination of OXC, S-Lic and R-Lic concentrations in beagle dog plasma.