Simultaneous determination of imperatorin and its metabolite xanthotoxol in rat plasma and urine by LC-MS/MS and its application to pharmacokinetic studies

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• 作者：Lien Ngo^a ; Phuong Tran^a ; Seong-Ho Ham^b ; Jung-Hee Cho^b ; Hea-Young Cho^c ; ^{hycho@cha.ac.kr} ; Yong-Bok Lee^a ; ^{leeyb@chonnam.ac.kr}
• 关键词：Imperatorin ; Xanthotoxol ; Metabolite ; LC&ndash ; MS/MS ; Pharmacokinetics
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：1044-1045
• 期：Complete
• 页码：30-38
• 全文大小：1223 K
• 卷排序：1044

文摘

An LC–MS/MS method for the simultaneous determination of IMP and XAN in rat plasma and urine was developed and validated. Chromatograms show good resolution with LLOQ of 0.25 ng/mL for IMP in rat plasma and urine, and 1 ng/mL for XAN in rat urine. Three-fold higher sensitivity obtained with the base peak [M – C2H5 + H]+ than the molecular ion peak [M + H]+ of IMP. The method was applied for a PK study of IMP after a dose of 3 mg/kg body weight to rats. The metabolite kinetics of IMP to XAN in rat urine after administration of IMP was investigated for the first time.