Evaluation of kudzu root extract-induced hepatotoxicity

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• 作者：Dengyuan Wang^a ; Liang Qiu^b ; Xiaoli Wu^b ; Hua Wei^c ; Feng Xu^a ; ^{ziwu211@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Acat2 ; cholesterolacyl transferase 2 ; ALT ; alanine aminotransferase ; AST ; aspartate aminotransferase ; Bax ; Bcl-2-associated x protein ; BUN ; blood urea nitrogen ; CREA ; creatinine ; DBIL ; direct bilirubin ; DMEM ; Dulbecco&rsquo ; s modified eagle medium ; Gclc ; glutamate-cysteine ligase catalytic subunit ; GLU ; glucose ; Ho-1 ; heme oxygenase 1 ; LDH ; lactate dehydrogenase ; LDL ; low density lipoprotein ; mRNA ; messenger RNA ; Mt ; metallothionein ; Mt1 ; metallothionein-1 ; Nrf2 ; nuclear factor E2-related factor
• 刊名：Journal of Ethnopharmacology
• 出版年：2015
• 出版时间：24 December 2015
• 年：2015
• 卷：176
• 期：Complete
• 页码：321-326
• 全文大小：1629 K

文摘

Kudzu root, the root of Pueraria lobata (Willd.) Ohwi, has been used as food and medicine for centuries, but few studies indicate that kudzu root may cause liver damage.

Aim of study

We studied the hepatotoxicity of kudzu root extract in mice, HepG2 cells and mice hepatocytes.

Materials and methods

Mice were administrated with kudzu root extract (10 mg/day) for 4 weeks, and then the biochemical analysis and histopathological changes were carried out. To explore the potential mechanism by which kudzu root extract-induced hepatotoxicity, HepG2 cells and mice hepatocytes were co-cultured with kudzu root extract or puerarin, which is a kudzu root isoflavone, for 2 h.

Results

The increase of serum ALT and AST and histopathological changes in treated mice revealed that kudzu root extract was hepatotoxic. The increase of LDH leakage for HepG2 cells and mice hepatocytes further confirmed hepatotoxicity of kudzu root extract. Kudzu root extract and puerarin significantly up-regulated Mt1 mRNA involved in the acute phase response and Bax which is crucial for apoptosis. Gclc, Nrf2 and Ho-1 mRNA expressions did not change in treatment group.

Conclusions

Kudzu root extract may be hepatotoxic and caution may be required for its use.