Tumor Angiogenesis in Breast Cancer: Pericytes and Maturation Does Not Correlate With Lymph Node Metastasis and Molecular Subtypes

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• 作者：Shreya Shrivastav¹ ; Amanjit Bal¹ ; ^{docaman5@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Gurpreet Singh² ; Kusum Joshi¹
• 关键词：Angiogenesis ; Breast cancer ; Microvessel density ; Microvessel pericyte coverage ; Proliferating capillary index
• 刊名：Clinical Breast Cancer
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：16
• 期：2
• 页码：131-138
• 全文大小：2966 K

文摘

Angiogenesis, traditionally assessed by microvessel density (MVD), does not give an indication of the functional status of the tumor neovasculature. The structural and functional stability of the tumor vasculature and its potential clinical relevance in breast cancer was evaluated.

Materials and Methods

In invasive breast cancer, immunostaining of endothelial cells and pericytes was performed using anti-CD34 and anti–platelet-derived growth factor receptor-β antibody, respectively. Double immunostaining for the proliferating capillary index (PCI) and microvessel pericyte coverage index (MPI) was performed with CD34/Ki-67 and CD34/smooth muscle actin.

Results

The mean MVD of 145 vessels/mm² was significantly greater in grade 3 tumors (P = .018) and in necrotic tumors (P = .022). The PCI ranged from 0% to 17.14% (mean, 4.37%) and was associated with a high proliferative index in tumor tissue (P = .044). The MPI ranged from 13.09% to 88.18% (mean, 41.35%), indicating the stability of the tumor vasculature. However, it was not significantly associated with the tumor size, tumor grade, lymph node metastasis, proliferative index, or molecular subtypes.

Conclusion

MVD remains the angiogenesis-related parameter associated with tumor grade and necrosis. The PCI was the only functional parameter of angiogenesis associated with a poor prognostic indicator. The MPI did not show any correlation with the known prognostic and predictive factors.