On the Use of Thiol-modifying Agents to Determine Channel Topology

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• 作者：HOLMGREN ; M. ; LIU ; Y. ; XU ; Y. ; YELLEN ; G.
• 关键词：Site-directed mutagenesis ; chemical modification ; thiol reagents ; methane thiosulfonate ; membrane protein topology ; potassium channels
• 刊名：Neuropharmacology
• 出版年：1996
• 出版时间：1996
• 年：1996
• 卷：35
• 期：7
• 页码：797-804
• 全文大小：599 K

文摘

A powerful tool in the study of cloned ion channels is the combined use of site-directed mutagenesis and chemical modification. Site-directed mutagenesis is used to introduce new cysteine residues at specific positions in a channel protein, and chemical modification by thiol-specific reagents is then used to assess the exposure of the introduced cysteines. This method has been used to assess secondary structure, membrane topology and conformational changes. We report that one commonly used, charged reagent (MTSEA; aminoethyl methanethiosulfonate) can cross the membrane quite readily. We also find that other reagents that are quite membrane-impermeant can cross the membrane when patches are electrically leaky. Both of these undesired effects can be controlled by the use of a thiol scavenger. These findings argue for caution in the use of modifying reagents to determine the membrane topology of channels and other membrane proteins. Copyright © 1996 Elsevier Science Ltd