A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris
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- 作者：Haifeng Hang ; Xinhao Ye ; Meijin Guo ; Ju Chu ; Yingping Zhuang ; Ming Zhang ; Siliang Zhang
- 关键词：Pichia pastoris ; Phytase ; Glucose ; Feeding strategy
- 刊名：Enzyme and Microbial Technology
- 出版年：2009
- 出版时间：6 April 2009
- 年：2009
- 卷：44
- 期：4
- 页码：185-188
- 全文大小：291 K
文摘
The methylotrophic yeast Pichia pastoris has become a popular host for the production of recombinant protein under the tight control of methanol-induced alcohol oxidase promoter (PAOX1), which is, however, strongly repressed by glucose at the transcription level. A real-time parameter-based controlled glucose feed strategy (rpcGFS) is developed here to realize Pichia high-cell density culture. It allows for the high-level production of recombinant phytase by P. pastoris YY113 using glucose as the growth substrate instead of glycerol. As a result, high phytase production (2200 FTU phytase ml−1) and yield (0.25 FTU ml−1[phytase] g−1[methanol]) are achieved successfully, respectively, after 100 h methanol induction by this novel simple fermentation strategy.