Defined subcomplexes of the A₁ ATPase from the archaeon Methanosarcina mazei Gö1: biochemical properties and redox regulation

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• 作者：Lemker ; Thorsten ; Grü ; ber ; Gerhard ; Schmid ; Roland ; Mü ; ller ; Volker
• 关键词：Archaeon ; A₁ ATPase ; Subcomplex ; Kinetic property ; Regulation ; Methanosarcina mazei Gö ; 1
• 刊名：FEBS Letters
• 出版年：2003
• 出版时间：June 5, 2003
• 年：2003
• 卷：544
• 期：1-3
• 页码：206-209
• 全文大小：150 K

文摘

The potential A₁ ATPase genes ahaA, ahaB, ahaC, ahaD, ahaE, ahaF, and ahaG from the anaerobic archaeon Methanosarcina mazei Gö1 were overexpressed in Escherichia coli DK8 (pTL2). An A₁ complex was purified to apparent homogeneity and shown by Western blot and N-terminal sequence analyses to contain subunits A, B, C, D, and F but to be devoid of subunits E and G. Further removal of subunit C was without effect on ATPase activity. The enzyme was most active at pH 5.2 and required bisulfite and acetate for maximal activity. Kinetic studies confirmed three new inhibitors for A₁ ATPases (diethylstilbestrol and its derivatives hexestrol and dienestrol) and identified redox modulation as a new type of regulation of archaeal A₁ ATPases.