Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution

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• 作者：Takuya Ogawa ; Tohru Yoshimura ; Hisashi Hemmi
• 关键词：Isoprenoid ; Archaea ; Methanogen ; Methanophenazine ; Prenyltransferase ; Geranylfarnesyl diphosphate synthase
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：26 February 2010
• 年：2010
• 卷：393
• 期：1
• 页码：16-20
• 全文大小：400 K

文摘

The gene of (all-E) geranylfarnesyl diphosphate synthase that is responsible for the biosynthesis of methanophenazine, an electron carrier utilized for methanogenesis, was cloned from a methanogenic archaeon Methanosarcina mazei Gö1. The properties of the recombinant enzyme and the results of phylogenetic analysis suggest that the enzyme is closely related to (all-E) prenyl diphosphate synthases that are responsible for the biosynthesis of respiratory quinones, rather than to the enzymes involved in the biosynthesis of archaeal membrane lipids, including (all-E) geranylfarnesyl diphosphate synthase from a thermophilic archaeon.