pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans
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- 作者：Mitchell T. Shea ; Mary E. Walter ; Nikolas Duszenko ; Anne-Lise Ducluzeau ; Jared Aldridge ; Shannon K. King ; Nicole R. Buan ; nbuan@unl.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Archaea ; Protein expression ; Methanogen ; Methanosarcina
- 刊名：Plasmid
- 出版年：2016
- 出版时间：March-May 2016
- 年：2016
- 卷：84-85
- 期：Complete
- 页码：27-35
- 全文大小：678 K
文摘
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We have created a suite of user-friendly plasmids for methanogens.
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The new plasmids are now compatible with ligation-independent cloning.
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We validated plasmids for markerless gene deletion.
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Plasmids were used to express a native and his-tagged reporter gene.