Aquamethemoglobin reduction by sodium n-dodecyl sulfate via coordinated water oxidation

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• 作者：Moosavi-Movahedi ; A.A. ; Dayer ; M.R. ; Norouzi ; P. ; Shamsipur ; M. ; Yeganeh-faal ; A. ; Chaichi ; M.J. ; et. al.
• 关键词：Methemoglobin reduction ; SDS ; Cyclic voltammetry ; Chemiluminescence
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2003
• 出版时间：July 1, 2003
• 年：2003
• 卷：30
• 期：1-2
• 页码：139-146
• 全文大小：265 K

文摘

The influence of sodium n-dodecyl sulfate (SDS) on hemoglobin reduction has been studied in the presence of 100 mM phosphate buffer (pH 7.0) using spectrophotometry, cyclic voltammetry and chemiluminescence (CL) methods. Spectroscopic studies have revealed that the SDS concentration up to 1 mM reduces the methemoglobin (metHb) to its deoxy form. Such a redox reaction is believed to occur by water oxidation of the sixth coordination in aquametHb. The proposed mechanism is supported by a CL method which monitors H₂O₂ during the conversion of metHb to its deoxy form by addition of SDS at low concentrations (up to 1 mM).