Plasma lipid oxidation induced by peroxynitrite, hypochlorite, lipoxygenase and peroxyl radicals and its inhibition by antioxidants as assessed by diphenyl-1-pyrenylphosphine
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- 作者：Mayuko Moritaa ; b ; Yuji Naitoa ; Toshikazu Yoshikawab ; Etsuo Nikia ; c ; etsuo-niki@aist.go.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：AAPH ; 2 ; 2&prime ; -azobis(2-amidinopropane) dihydrochloride ; DPPP ; diphenyl-1-pyrenylphosphine ; FI ; fluorescence intensity ; LOX ; lipoxygenase ; MeO-AMVN ; 2 ; 2&prime ; -azobis-(4-methoxy-2 ; 4-dimethyllvaleronitrile) ; NDGA ; nordihydroguaiaretic acid ; SIN-1 ; 3-morpholinosydnonimine
- 刊名：Redox Biology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：127-135
- 全文大小：2267 K
文摘
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Multiple oxidants oxidize plasma to give lipid hydroperoxides.
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Peroxyl radicals, peroxynitrite, hypochlorite and lipoxygenase were studied.
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Plasma lipid hydroperoxides react with DPPP to give highly fluorescent DPPP oxide.
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Production of lipid hydroperoxide was followed by increase in fluorescence intensity.
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This system was applied to assessment of antioxidant capacity with microplate reader.